E11 Bio - Scientist, In vivo circuit mapping

E11 Bio is on a mission to advance connectomics towards whole brain scale for humans and other mammals. We are a collaborative and interdisciplinary team of scientists and engineers engaged in a neuroscience moonshot project to develop a radical technology platform for scalable mammalian brain mapping. E11 Bio is a non-profit Convergent Research focused research organization located in Alameda, California, pursuing blue-skies neuroscience research yet operating like a nimble, tight-knit start-up. Read our technology roadmap.

E11 is seeking a circuit mapping scientist to help engineer viral and cellular labels for a moonshot effort making whole-brain connectomics feasible in humans and mice to revolutionize neuroscience and medicine.

Your primary mission as a Scientist, circuit mapping is to design, execute on, and optimize our in vivo barcode delivery, the heart of E11's technology stack.

About the Role:

In your first month, you’ll shadow and work with our lead barcoding scientist on ongoing experiments, learning the why and the how of our cellular labeling approaches. This includes our cloning workflow, our AAV prep, and the organization of our in vivo work. You will have launched your first labeling experiments with the help of our in vivo surgeon, identified parts of the process in need of an overhaul, and familiarize yourself with E11’s cross-functional teams.

By 2 months, you’ll have imaged, analyzed, and communicated out your first set of barcoding results. You’ll have owned at least 2 in vivo experiments from start to finish. In collaboration with our lead barcoding scientist, you’ll have initiated a plan or several to expand our ability to label & read out barcoded neural circuits.

At the 3 month mark, you will fully own coordination of circuit labeling at E11 and hand-offs to other team members. You’ll know our viral and genetic tools backwards and forwards, and you’ll be driving your project for neural circuit labeling forward, executing on design, in vivo delivery, readout, and analysis.

And you:

You are passionate about moonshot neuroscience, and want to broadly accelerate science through long-term commitment to a high-impact project with a tight-knit team.

You have experience with mouse genetics and/or in vivo viral labeling of neural circuits, and are excited to add basic Python and command line usage to your skillset.

You have experience with biological barcoding systems (e.g. MAPseq; rabies-based; Brainbow; in vivo pooled screens; lineage tracing).

You have experience collaborating on time-sensitive projects with large numbers of experimental conditions, often with overlapping time-courses. Additional points for experience collaborating in a team structure across multiple projects.

Responsibilities:

• Design, build, and test viral and genetic protein barcoding systems for whole-mouse brain labeling
• Work closely with imaging and computational collaborators to co-design of barcodes with excellent signal and highly-efficient encoding/decoding of information
• Direct stereotaxic surgeries, maintain records, and analyze outcomes from stereotaxic and systemic delivery systems of barcoding virus (incorporating feedback from E11 team and external collaborators as needed)
  
  
  

We Offer:

• An opportunity to help change the world and work with some of the most talented and collaborative experts across scientific fields
• Excellent medical, dental, and vision insurance through a PPO plan; parental leave
• Generous time off + paid holidays
• Wellness allowance for fitness and wellness activities
  
  
  

E11 Bio, LLC is an Equal Employment Opportunity employer that proudly pursues and hires a diverse workforce. We do not make hiring or employment decisions on the basis of race, color, religion or religious belief, ethnic or national origin, nationality, sex, gender, gender-identity, sexual orientation, disability, age, military or veteran status, or any other basis protected by applicable local, state, or federal laws or prohibited by Company policy. We strive for a healthy and safe workplace and strictly prohibit harassment of any kind.